首页 全所PI名录
  • 豆晓阳
  • 研究员，研究组长，博士生导师
  • E-mail: xiaoyang.dou@sibcb.ac.cn
  • 实验室主页: 
    个人简介：

  • 2011年毕业于河北经贸大学； 2018年毕业于中科院计算生物学研究所，获计算生物学博士学位；2018年12月至2024年6月于芝加哥大学从事博士后研究； 拟于2024年7月起任中科院分子细胞科学卓越创新中心 (生物化学与细胞生物学研究所) 研究员，研究组长，博士生导师。

    社会任职：
    研究方向：
  • RNA修饰与染色质调控、计算生物学
    研究工作：

  • 非编码RNA及其化学修饰是基因调控网络中的核心调节因子，它们在RNA与蛋白质、DNA及其他RNA等生物大分子的相互作用中起着关键作用。这种相互作用对调节染色质重塑和基因转录活化至关重要， 从而影响一系列生物学过程。通过结合表观转录组学、表观遗传学、基因组学和计算生物学等交叉学科的优势，采用干湿实验相结合的研究方法，我们将：

    （1）深入探索非编码RNA及其化学修饰在染色质重塑和基因转录活化中的调控模式、机制和生物学功能。

    （2）解析非编码RNA及其化学修饰在神经退行性疾病和癌症等人类重大疾病中异常代谢的成因与后果，及其在免疫治疗中的作用与机制。

    （3）通过数据整合和深度数据挖掘，研究非编码RNA的命运决定模式。

    我们的研究目标是深化对具有调控功能的非编码RNA及其化学修饰的理解，拓展其应用潜力，为疾病的预防、诊断和治疗提供新的分子靶点和策略。欢迎加入我们！

    承担科研项目情况：
    代表论著：
    1. Dou, X.*, Xiao, Y. *, Shen, C. *, Wang, K. *, Wu, T., Liu, C., Li, Y., Yu, X., Liu, J., Dai, Q., Pajdzik, K., Ye, C., Ge, R., Gao, B., Yu, J., Sun, S., Chen, M., Chen, J., & He, C. (2023). RBFOX2 recognizes N6-methyladenosine to suppress transcription and block myeloid leukaemia differentiation. Nature Cell Biology, 25(9), 1359–1368. 
    2. Li, Y. *, Dou, X. *, Liu, J. *, Xiao, Y. *, Zhang, Z., Hayes, L., Wu, R., Fu, X., Ye, Y., Yang, B., Ostrow, L. W., He, C., & Sun, S. (2023). Globally reduced N6-methyladenosine (m6A) in C9ORF72-ALS/FTD dysregulates RNA metabolism and contributes to neurodegeneration. Nature Neuroscience, 26(8), 1328–1338.
    3. Wang, L.*, Dou, X.*, Chen, S.*, Yu, X., Huang, X., Zhang, L., Chen, Y., Wang, J., Yang, K., Bugno, J., Pitroda, S., Ding, X., Piffko, A., Si, W., Chen, C., Jiang, H., Zhou, B., Chmura, S. J., Luo, C., Liang, H. L., … Weichselbaum, R. R. (2023). YTHDF2 inhibition potentiates radiotherapy antitumor efficacy. Cancer Cell, 41(7), 1294–1308.e8.
    4. Dou, X.*, Huang, L.*, Xiao, Y.*, Liu, C., Li, Y., Zhang, X., Yu, L., Zhao, R., Yang, L., Chen, C., Yu, X., Gao, B., Qi, M., Gao, Y., Shen, B., Sun, S., He, C., & Liu, J. (2023). METTL14 is a chromatin regulator independent of its RNA N6-methyladenosine methyltransferase activity. Protein & Cell, 14(9), 683–697.
    5. He, P. C.*, Wei, J.*, Dou, X.*, Harada, B. T., Zhang, Z., Ge, R., Liu, C., Zhang, L. S., Yu, X., Wang, S., Lyu, R., Zou, Z., Chen, M., & He, C. (2023). Exon architecture controls mRNA m6A suppression and gene expression. Science, 379(6633), 677–682. 
    6. Liu, J.*, Dou, X.*, Chen, C.*, Chen, C., Liu, C., Xu, M. M., Zhao, S., Shen, B., Gao, Y., Han, D., & He, C. (2020). N6-methyladenosine of chromosome-associated regulatory RNA regulates chromatin state and transcription. Science, 367(6477), 580–586. 
    7. Dou, X.*, Boyd-Kirkup, J. D.*, McDermott, J.*, Zhang, X.*, Li, F., Rong, B., Zhang, R., Miao, B., Chen, P., Cheng, H., Xue, J., Bennett, D., Wong, J., Lan, F., & Han, J. J. (2019). The strand-biased mitochondrial DNA methylome and its regulation by DNMT3A. Genome Research, 29(10), 1622–1634.
    8. Green, C. D.*, Huang, Y.*, Dou, X.*, Yang, L., Liu, Y., & Han, J. J. (2017). Impact of Dietary Interventions on Noncoding RNA Networks and mRNAs Encoding Chromatin-Related Factors. Cell Reports, 18(12), 2957–2968.
    9. Guo, J., Zou, Z., Dou, X., Zhao, X., Wang, Y., Wei, L., Pi, Y., Wang, Y., He, C., & Guo, S. (2024). Zebrafish Mbd5 binds to RNA m5C and regulates histone deubiquitylation and gene expression in development metabolism and behavior. Nucleic Acids Research, gkae093. 
    10. Wu, T., Cheng, A. Y., Zhang, Y., Xu, J., Wu, J., Wen, L., Li, X., Liu, B., Dou, X., Wang, P., Zhang, L., Fei, J., Li, J., Ouyang, Z., & He, C. (2024). KARR-seq reveals cellular higher-order RNA structures and RNA-RNA interactions. Nature Biotechnology, 10.1038/s41587-023-02109-8. 
    11. Xiao, Y., Chen, Y. M., Zou, Z., Ye, C., Dou, X., Wu, J., Liu, C., Liu, S., Yan, H., Wang, P., Zeng, T. B., Liu, Q., Fei, J., Tang, W., & He, C. (2024). Profiling of RNA-binding protein binding sites by in situ reverse transcription-based sequencing. Nature Methods, 21(2), 247–258.
    12. Wang, L., Si, W., Yu, X., Piffko, A., Dou, X., Ding, X., Bugno, J., Yang, K., Wen, C., Zhang, L., Chen, D., Huang, X., Wang, J., Arina, A., Pitroda, S., Chmura, S. J., He, C., Liang, H. L., & Weichselbaum, R. (2023). Epitranscriptional regulation of TGF-β pseudoreceptor BAMBI by m6A/YTHDF2 drives extrinsic radioresistance. The Journal of Clinical Investigation, 133(24), e172919.
    13. Zhang, L., Dou, X., Zheng, Z., Ye, C., Lu, T. X., Liang, H. L., Wang, L., Weichselbaum, R. R., & He, C. (2023). YTHDF2/m6 A/NF-κB axis controls anti-tumor immunity by regulating intratumoral Tregs. The EMBO Journal, 42(15), e113126.
    14. Dai, Q., Zhang, L. S., Sun, H. L., Pajdzik, K., Yang, L., Ye, C., Ju, C. W., Liu, S., Wang, Y., Zheng, Z., Zhang, L., Harada, B. T., Dou, X., Irkliyenko, I., Feng, X., Zhang, W., Pan, T., & He, C. (2023). Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution. Nature Biotechnology, 41(3), 344–354.
    15. Chokkalla, A. K., Pajdzik, K., Dou, X., Dai, Q., Mehta, S. L., Arruri, V., & Vemuganti, R. (2023). Dysregulation of the Epitranscriptomic Mark m1A in Ischemic Stroke. Translational Stroke Research, 14(6), 806–810.
    16. Wei, J., Yu, X., Yang, L., Liu, X., Gao, B., Huang, B., Dou, X., Liu, J., Zou, Z., Cui, X. L., Zhang, L. S., Zhao, X., Liu, Q., He, P. C., Sepich-Poore, C., Zhong, N., Liu, W., Li, Y., Kou, X., Zhao, Y., … He, C. (2022). FTO mediates LINE1 m6A demethylation and chromatin regulation in mESCs and mouse development. Science, 376(6596), 968–973.
    17. Chen, C., Liu, W., Guo, J., Liu, Y., Liu, X., Liu, J., Dou, X., Le, R., Huang, Y., Li, C., Yang, L., Kou, X., Zhao, Y., Wu, Y., Chen, J., Wang, H., Shen, B., Gao, Y., & Gao, S. (2021). Nuclear m6A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos. Protein & Cell, 12(6), 455–474.
    18. Hao, Z., Wu, T., Cui, X., Zhu, P., Tan, C., Dou, X., Hsu, K. W., Lin, Y. T., Peng, P. H., Zhang, L. S., Gao, Y., Hu, L., Sun, H. L., Zhu, A., Liu, J., Wu, K. J., & He, C. (2020). N6-Deoxyadenosine Methylation in Mammalian Mitochondrial DNA. Molecular Cell, 78(3), 382–395.e8.
    19. Liu, C., Cui, X., Zhao, B. S., Narkhede, P., Gao, Y., Liu, J., Dou, X., Dai, Q., Zhang, L. S., & He, C. (2020). DNA 5-Methylcytosine-Specific Amplification and Sequencing. Journal of the American Chemical Society, 142(10), 4539–4543.
    20. Cheng, H.*, Dou, X.*, & Han, J. D. (2016). Understanding super-enhancers. Science China. Life Sciences, 59(3), 277–280.
    21. Liu, P., Dou, X., Liu, C., Wang, L., Xing, C., Peng, G., Chen, J., Yu, F., Qiao, Y., Song, L., Wu, Y., Yue, C., Li, J., Han, J. D., Tang, K., & Jing, N. (2015). Histone deacetylation promotes mouse neural induction by restricting Nodal-dependent mesendoderm fate. Nature Communications, 6, 6830.
    获奖及荣誉：
    研究组成员：
Copyright 2017-　中国科学院分子细胞科学卓越创新中心(生物化学与细胞生物学研究所)　版权所有
备案号: 沪ICP备2021025838号 　　地址：上海岳阳路320号
　邮编：200031 传真：021-54921011 所长信箱：sibcb@sibcb.ac.cn