1998年毕业于中国医科大学七年制外文系，获得医学学士学位;2003年3月毕业于日本神户大学，获得医学博士学位;2003年6月至2008年7月在美国哈佛医学院细胞生物系从事博士后研究;2008年7月起任中国科学院上海生命科学研究院生物化学与细胞生物学研究所研究员，博士生导师和研究组长。

　　真核细胞中，核膜将贮存遗传信息的DNA与翻译遗传信息的核糖体分隔在细胞核与细胞质中。由RNA介导的遗传信息跨核膜传递为所有真核生物生命活动所必需。新生pre-mRNA在细胞核内完成一系列复杂加工后，成熟mRNA被转运到细胞质中进行翻译。mRNA出核转运与pre-mRNA加工过程相互偶联，确保遗传信息的精准表达。细胞核中普遍存在RNA质量监控系统，可快速识别并降解携带错误遗传信息的或异常mRNA，从而保证遗传信息的准确传递。新生转录本被快速、准确地分选到出核或降解通路是遗传信息高效、精准传递的基本保证，具有重要生物学意义。本实验室自2008年成立以来，主要围绕“新生转录本出核和降解的命运决定与调控”这一基因表达领域的关键科学问题，系统性地开展mRNA出核与加工的复杂关联、RNA的质量控制和核内降解及其调控两个方向的研究工作。具体如下。

　　利用分子与细胞生物学、生物化学、化学生物学及多种组学研究手段及技术，重点研究：

　　1、mRNA出核与pre-mRNA加工的复杂关联;

　　2、RNA的质量控制和核内降解及其调控;

　　3、细胞核亚结构调控RNA出核或降解的生理病理机制。

1. Wang CS#, Xu L#, Du C#, Yun H#, Wang K#, Liu H, Ye ML*, Fan J*, Zhou Y*, and Cheng H*. CDK11 requires a critical activator SAP30BP to regulate pre-mRNA splicing. EMBO J (2023) 114051R1
2. Fu X#, Li S#, Jia M#, Xu B, Yang L, Ma R, Cheng H, Yang W*, Hu P*. Myogenesis controlled by a long non-coding RNA 1700113A16RIK and post-transcriptional regulation. Cell Regeneration (2022)11(13), DOI: 10.1186/s13619-022-00114-x
3. Chen C#, Tan M, Wu Z, Zhang Y, He F, Lu Y, Li S, Cao M, Li G, Wu J, Cheng H*, Lei M*. Structural and functional insights into R-loop prevention and mRNA export by budding yeast THO-Sub2 complex. Science Bulletin (2021) 66(23): 2347-2352
4. Wang YM#, Fan J#*, Wang JS, Zhu Y, Xu L, Tong D, Cheng H*. ZFC3H1 prevents RNA trafficking into nuclear speckles through condensation. Nucleic Acids Research (2021) 49(18): 10630-10643
5. Wei MY#, Huang JS, Li GW, Jiang BW, Cheng H, Liu XY, Jiang XY, Zhang X, Yang L, Bao L* ,  Wang B*.Axon-enriched lincRNA ALAE is required for axon elongation via regulation of local mRNA translation. Cell Reports (2021) 35, 109053
6. Cheng LC#, Zheng DH, Zhang Q, Guvenek A, Cheng H, and Tian B*. Alternative 3 UTRs play a widespread role in translation-independent mRNA association with the endoplasmic reticulum Cell Reports (2021) 36, 109407
7. Wang JS#, Cheng H*. Out or decay: fate determination of nuclear RNAs. Essays in Biochemistry (2020), 64(6): 895-905
8. Wang LT^#, Fan J^#, Han LL^#, Qi HN^#, Wang YM, Wang HY, Chen SL, Du L, Li S, Zhang YB, Tang W，Ge GX, Pan WJ*, Hu P*, Cheng H*. The micropeptide LEMP plays an evolutionarily conserved role in myogenesis. Cell Death Dis (2020) 11(5):357 DOI: 10.1038/ s41419 -020- 2570-5
9. Zhao K^#, Cheng S^#, Miao N^#, Xu P^#, Lu X^#, Zhang Y^#, Wang M, Ouyang X, Yuan X, Liu W, Lu X, Zhou P, Gu J, Zhang Y, Qiu D, Jin Z, Su C, Peng C, Wang JH, Dong MQ, Wan Y, Ma J, Cheng H, Huang Y*, Yu Y*.A Pandas complex adapted for piRNA-guided transcriptional silencing and heterochromatin formation.  Nature Cell Biology (2019) 21(10):1261-1272.
10. Shin J, Cheng H*, Tian B*. New means to an end: mRNA export activity impacts alternative polyadenylation. Transcription (2019) DOI: 10.1080/21541264.2019.1658557
11. Chen SL^#, Wang RJ^#, Zheng DH^#, Zhang H^#, Chang XY, Wang K, Li WC, Fan J, Tian B*, Cheng H*. The mRNA export receptor NXF1 coordinates transcriptional dynamics, alternative polyadenylation, and mRNA export. Mol Cell (2019) 74(1):118-131
12. Wang JS^#, Chen JY^#, Wu GF^#, Zhang HL^#, Du X^#, Chen SL, Zhang L, Wang K, Fan J, Gao SX, Wu XD, Zhang SX, Kuai B, Zhao P, Chi BK, Wang LT, Li GH, Wong CCL, Zhou Y*, Li JS*, Yun CH*, Cheng H*. NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction. Genes Dev (2019) 33(9-10):536-549
13. Wang K, Yin CP, Du X, Chen SL, Wang JS, Zhang L, Wang LT, Yu Y, Chi BK, Shi M, Wang CS, Reed R, Zhou Y, Huang J, Cheng H*. A U2 snRNP independent role of SF3b in promoting mRNA export. Proc Natl Acad Sci USA (2019) 116(16):7837-7846
14. Fan J, Wang K, Du  X, Wang JS, Chen SL, Wang YM, Shi M, Zhang L, Wu XD, Zheng DH, Wang CS, Wang LT, Tian B, Li GH, Zhou Y, Cheng H*. ALYREF links 3’-end processing to nuclear export of non-polyadenylated mRNAs. EMBO J (2019) 38(9)DOI: 10.15252/embj.201899910
15. Wang K, Wang LT, Wang JS, Chen SL, Shi M, Cheng H*. Intronless mRNAs transit through nuclear speckles to gain export competence. J Cell Biol (2018) 217: 3912-3929. (Recommended by F1000Prime)
16. Fan J^#, Kuai B^#, Wang K^#, Wang LT, Wang YM, Wu XD, Chi BK, Li GH, Cheng H*. mRNAs are sorted for export or degradation before passing through nuclear speckles. Nucleic Acids Res (2018) 46: 8404-8416
17. Lan PF^#, Tan M^#, Zhang YB^#, Niu SS, Chen J, Shi SH, Qiu SW, Wang XJ, Peng XD, Cai G, Cheng H, Wu J*, Li GH*, Lei M*. Structural insight into precursor tRNA processing by yeast ribonuclease P. Science (2018) 362(6415). DOI:10.1126/science.aat6678
18. Wang K, Shi M, Cheng H*. Microinjection and fluorescence in situ hybridization assay for studying mRNA export in mammalian cells. Methods Mol Biol(2017) 1648:95-102
19. Shi M, Zhang H, Wu XD, He ZS, Wang LT, Yin SY, Tian B, Li GH*, Cheng H*. ALYREF mainly binds to the 5’ and the 3’ regions of the mRNA in vivo. Nucleic Acids Res (2017) 45: 9640-9653
20. Fan J^#, Kuai B^#, Wu GF, Wu XD, Chi BK, Wang LT, Wang K, Shi ZB, Zhang H, Chen S, He ZS, Wang SY, Zhou ZC, Li GH*, Cheng H*.Exosome cofactor hMTR4 competes with export adaptor ALYREF to ensure balanced nuclear RNA pools for degradation and export. EMBO J (2017) 36: 2870-2886
21. Zhang DL, Chen DF, Cao LR, Li GH*, Cheng H*. The effect of codon mismatch on the protein translation system. PLoS One (2016) 11(2):e0148302. DOI:10.1371/journal.pone.0148302
22. Shi M^#, Zhang H^#, Wang LT^#, Zhu CL, Sheng K, Du YH, Wang K, Dias A, Chen S, Whitman M, Wang ED, Reed R *, Cheng H*. Premature termination codons are recognized in the nucleus in a reading-frame dependent manner. Cell Discov (2015) 1, 15001. DOI: 10.1038/celldisc.2015.1
23. Chi BK, Wang K, Du YH, Gui B, Chang XY, Wang LT, Fan J, Chen S, Wu XD, Li GH*, Cheng H*. A sub-element in PRE enhances nuclear export of intronless mRNAs by recruiting the TREX complex via ZC3H18.Nucleic Acids Res (2014) 42: 7305-18
24. Chi BK^#, Wang QL^#, Wu GF, Tan M, Wang LT, Shi M, Chang XY, Li GH, Cheng H*. Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA. Nucleic Acids Res (2013) 41: 1294-1306
25. Liu HJ^#, Wu ZT^#, Shi XL^#, Li WZ^#, Liu C, Wang DK, Ye XY, Liu L, Na J*, Cheng H*, Chen LY*. Atypical PKC, regulated by Rho GTPases and Mek/Erk, phosphorylates Ezrin during eight-cell embryo compaction. Dev Biol (2013) 375: 13-22
26. Wu ZT, Yang M, Liu HJ, Guo HC, Wang Y, Cheng H, Chen LY*. Role of nuclear receptor coactivator 3 (Ncoa3) in pluripotency maintenance. J Biol Chem (2012) 287: 38295-304
27. Cheng H, Dufu K, Valencia P, Reed R*. mRNA Export. Encyclopedia of Life Sciences (eLS) (2007) DOI: 10.1002/9780470015902.a0005977.pub2
28. Cheng H, Dufu K, Lee CS, Hsu J. Dias A, Reed R*. Human mRNA export machinery recruited to the 5′ end of mRNA. Cell (2006) 127: 1389-400
29. Reed R*, Cheng H. TREX, SR proteins and export of mRNA. Curr Opin Cell Biol (2005) 17: 269-73
30. Masuda S, Das R, Cheng H, Hurt E, Dorman N, Reed R*. Recruitment of the human TREX complex to mRNA during splicing. Genes Dev (2005) 19: 1512-7
31. Cheng H^#, Sugiura R^#, Wu WL, Fujita M, Lu YB, Sio SO, Kawai R, Takegawa K, Shuntoh H, Kuno T*. Role of the Rab GTP-binding protein Ypt3 in the fission yeast exocytic pathway and its connection to calcineurin function. Mol Biol Cell (2002) 13: 2963-76
32. Lu YB, Sugiura R, Yada T, Cheng H, Sio SO, Shuntoh H, Kuno T*. Calcineurin is implicated in the regulation of the septation initiation network in fission yeast. Cover story. Genes to Cells (2002) 7: 1009-19

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