首页 全所PI名录
  • 陈玲玲
  • 研究员,研究组长,博士生导师
  • E-mail: linglingchen@@sibcb.ac.cn
  • 实验室主页: www.chenlab-ncrna.com
    个人简介:
  •   2009年毕业于美国康涅狄格大学医学院获生物医学博士学位,同时获商学院工商管理学硕士学位。同年5月作为独立PI获得Connecticut Stem Cell Seed Award研究经费资助,受聘于康涅狄格大学干细胞学院从事博士后研究,并于2010年5月任助理教授。2011年1月任上海生化与细胞所研究员。目前担任Cell、Mol Cell等多个国际期刊编委、美国冷泉港实验室非编码RNA会议、国际RNA协会年会等多个会议的大会主席。2017年入选霍华德·休斯医学院研究所(HHMI)国际研究学者、国家杰出青年科学基金等资助。曾获中国青年科技奖特别奖、科学探索奖、谈家桢生命科学创新奖、全球华人生物学家协会CBIS青年研究员奖和中国青年女科学家奖等荣誉。

    社会任职:
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    研究方向:
  •   RNA加工代谢与功能机制
    研究工作:
  •   人类基因组测序计划发现多于98%的转录基因组序列为非编码RNA(ncRNA)。这些ncRNA包括人们所熟知的housekeepingncRNA(如tRNAs, rRNAs和snRNAs等)、小RNA(如miRNAs和piRNAs等),以及上万条长非编码RNA(lncRNA,>200nt)。最近几年来的研究表明lncRNAs广泛参与一系列细胞的重要功能调控,包括细胞核亚结构的形成、基因表达的遗传与表观遗传调控等。

      我们实验室主要研究哺乳动物细胞中lncRNAs的功能调控,包括新类型lncRNA分子的发掘、其加工成熟机制、以及它们在转录、染色体、细胞核亚结构和干细胞干性维持等方面的调控功能研究。未来五年,我们主要研究以下内容:

      1、通过转录组测序和计算生物学手段结合的方法,发掘和鉴定人类基因组中的新类型lncRNA分子

      2、利用分子生物学、细胞生物学和生物化学的手段,研究新lncRNA分子在基因表达调控中的作用机制

      3、采用基因组编辑和活细胞成像等技术手段,研究lncRNA在细胞核亚结构和染色体维持和调控过程中的功能

      4、以hESCs的多功能潜能性维持和分化为模型,研究lncRNA对人源胚胎干细胞的命运决定的调控

      研究成果将能够丰富我们对lncRNAs功能和机制的认识,也为hESCs的多功能性维持和命运改变提供一个全新水平的调控。

    承担科研项目情况:
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    代表论著:
    1. Li SQ#, Li X#, Xue W#, Zhang L#, Yang LZ, Cao SM, Lei YN, Liu CX, Guo SK, Shan L, Wu M, Tao X, Zhang JL, Gao X, Zhang J, Wei J, Li JS*, Yang L* and Chen LL*. 2020. Screening for functional circular RNAs using the CRISPR-Cas13 system. Nat Methods, in press.
    2. Guo CJ, Xu G and Chen LL*. 2020. Mechanisms of Long Noncoding RNA Nuclear Retention. Trends Biochem Sci, 45(11):947-960.
    3. Chen LL*. 2020. The expanding regulatory mechanisms and cellular functions of circular RNAs. Nat Rev Mol Cell Biol, 21(8):475-490.
    4. Guo CJ#, Ma XK#, Xing YH, Zheng CC, Shan L, Xu YF, Zhang J, Wang SH, Wang YM, Carmichael GG, Yang L and Chen LL*. 2020. Distinct processing of lncRNAs contributes to non-conserved functions in stem cells. Cell, 181(3):621-636. (Preview by Sharma H and Carninci P, Cell, 2020, 181(3):512-514).
    5. Yao RW, Liu CX and Chen LL*. 2020. Linking RNA processing and function. Cold Spring Harb Symp Quant Biol, 84:67-82.
    6. Yang LZ#, Wang Y#, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG and Chen LL*. 2019. Dynamic imaging of RNA in living cells by CRISPR-Cas13 system. Mol Cell, 76(6):981-997. (F1000 Prime recommendation and Spotlighted by Davis BJ and O’Connell MR, Mol Cell, 2020, 77:207-209).
    7. Yao RW#, Xu G#, Wang Y, Shan L, Luan PF, Wang Y, Wu M, Yang LZ, Xing YH, Yang L and Chen LL*. 2019. Nascent pre-rRNA sorting via phase separation drives the assembly of dense fibrillar components in the human nucleolus. Mol Cell, 76(5):767-783. (Featured article and Issue highlights; Preview by Lafontaine DL, Mol Cell, 2019, 76(5): 694-696; Research Highlights by Nat Chem Biol, 2019, 15:1029).
    8. Liu CX#, Li X#, Nan F#, Jiang S, Gao X, Guo SK, Xue W, Cui YG, Dong KG, Ding HH, Qu B, Shen N*, Yang L*and Chen LL*. 2019. Structure and degradation of circular RNAs regulate PKR activation in innate immunity. Cell, 177(4):865-880. (Preview by Wilusz JE, Cell, 2019, 177:797-799; Research highlights by Nat Rev Immunol, 2019, 19:351; Nat Rev Mol Cell Biol, 2019, 20:387; Science, 2019, 364:847; Cell Bioscience, 2019, 9:43-45; Bull Chin Acad Sci, 2019, 33:91-95; Recommended by F1000 Prime; News reports by Science News Magazine, LUPUS NEWS, Technologytimes, Medical Xpress).
    9. Yao RW#, Wang Y# and Chen LL*. 2019. Cellular functions of long noncoding RNAs. Nat Cell Biol, 21(5):542-551. (Editorial by Nat Cell Biol, 2019, 21(5):535).
    10. Wang Y#, Hu SB#, Wang MR#, Yao RW, Wu D, Yang L and Chen LL*. 2018. Genome-wide screening of NEAT1 regulators reveals cross-regulation between paraspeckles and mitochondria. Nat Cell Biol, 20(10):1145-1158. (News and Views by Fox AH, Nat Cell Biol, 2018, 20: 1108-1109).
    11. Xing YH and Chen LL*. 2018. Processing and roles of snoRNA-ended long noncoding RNAs. Crit Rev Biochem Mol Biol, 53(6):596-606.
    12. Li X, Yang L* and Chen LL*. 2018. The biogenesis, functions and challenges of circular RNAs. Mol Cell, 71(3):428-442.
    13. Xiang JF#, Yang Q#, Liu CX#, Wu M, Chen LL* and Yang L*. 2018. N6-methyladenosines modulate A-to-I RNA editing. Mol Cell, 69(1):126-135.
    14. Li X#, Liu CX#, Xue W#, Zhang Y, Jiang S, Yin QF, Wei J, Yao RW, Yang L* and Chen LL*. 2017. Coordinated circRNA biogenesis and function with NF90/NF110 in viral infection. Mol Cell, 67(2):214-227. (Free featured article; Highlighted by Editor’s Note and Meet the Author; Preview by Cadena CY and Hur S, Mol Cell, 2017, 67:163-164).
    15. Xing YH#, Yao RW#, Zhang Y#, Guo CJ, Jiang S, Xu G, Dong R, Yang L and Chen LL*. 2017. SLERT regulates DDX21-rings associated with Pol I transcription. Cell, 169(4):664-678. (Research highlights by Bull Chin Acad Sci, 2017, 31:152-155).
    16. Yang L* and Chen LL*. 2017. Enhancing the RNA engineering toolkit. Science, 358(6366):996-997.
    17. Wu H, Yang L* and Chen LL*. 2017. The Diversity of Long Noncoding RNAs and Their Generation. Trends Genet, 33(8):540-552.
    18. Chen LL* and Yang L*. 2017. AlUternative regulation for gene expression. Trends Cell Biol, 27(7):480-490.
    19. Dong R#, Ma XK#, Chen LL* and Yang L*. 2017. Increased complexity of circRNA expression during species evolution. RNA Biol, 14(8):1064-1074.
    20. Wu H#, Yin QF#, Luo Z#, Yao RW, Zheng CC, Zhang J, Xiang JF, Yang L and Chen LL*. 2016. Unusual processing generates SPA lncRNAs that sequester multiple RNA binding proteins. Mol Cell, 64(3):534-548. (Cover story and Issue highlights; Preview by Li RH and Fox AH, Mol Cell, 2016, 64:435-437).
    21. Chen LL*. 2016. The biogenesis and emerging roles of circular RNAs. Nat Rev Mol Cell Biol, 17(4):205-211. (Cover article).
    22. Zhang XO#, Dong R#, Zhang Y#, Zhang JL, Luo Z, Zhang J, Chen LL*and Yang L*. 2016. Diverse alternative back-splicing and alternative splicing landscape of circular RNAs. Genome Res, 26(9):1277-1287.
    23. Zhang Y#, Xue W#, Li X, Zhang J, Zhang JL, Yang L* and Chen LL*. 2016. The biogenesis of nascent circular RNAs. Cell Rep, 15(3):611-624.
    24. Chen LL*. 2016. Linking long noncoding RNA localization and function. Trends Biochem Sci, 41(9):761-772. (Series: Fresh Perspectives from Emerging Experts).
    25. Hu SB, Yao RW and Chen LL*. 2016. Shedding light on paraspeckle structure by super-resolution microscopy. J Cell Biol, 214(7):789-791.
    26. Hu SB, Xiang JF, Li X, Xu Y, Xue W, Huang M, Wong CC, Sagum CA, Bedford MT, Yang L, Cheng D* and Chen LL*. 2015. Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus. Genes Dev, 29(6):630-645. (Perspectives by Elbarbary RA and Maquat LE, Genes Dev, 2015, 29:687-689; Research highlights by Bull Chin Acad Sci, 2015, 29:108).
    27. Chen T#, Xiang JF#, Zhu S#, Chen S, Yin QF, Zhang XO, Zhang J, Feng H, Dong R, Li XJ, Yang L* and Chen LL*. 2015. ADAR1 is required for differentiation and neural induction by regulating microRNA processing in a catalytically independent manner. Cell Res, 25(4):459-476. (Highlighted by ESC& iPSC NEWS).
    28. Yin QF#, Hu SB#, Xu YF, Yang L, Carmichael GG and Chen LL*. 2015. SnoVectors for nucelar expression of RNA. Nucleic Acids Res, 43:e5.
    29. Chen LL* and Yang L*. 2015. Regulation of circRNA biogenesis. RNA Biol, 12(4):381-388.
    30. Xiang JF, Yang L and Chen LL*. 2015. The long noncoding RNA regulation at the MYC locus. Curr Opin Genet Dev, 33:41-48.
    31. Chen LL* and Yang L*. 2015. Gear up in circles. Mol Cell, 58(5):715-717.
    32. Zhang XO#, Wang HB#, Zhang Y, Lu X, Chen LL* and Yang L*. 2014. Complementary sequence-mediated exon circularization. Cell, 159(1):134-147. (Featured article; Preview by Vicens Q and Westhof E, Cell, 2014,159:13-14; Research highlights by Nat Rev Genetics, 2014, 15:717 and Bull Chin Acad Sci, 2014, 28:282-284; F1000 Prime recommendation).
    33. Xiang JF, Yin QF, Chen T, Zhang Y, Zhang XO, Wang HB, Ge JH, Lu XH, Yang L and Chen LL*. 2014. Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions in the MYC locus. Cell Res, 24(5):513-531. (Cover article; Research highlights by Younger ST and Rinn JL. Cell Res, 2014, 24:643-644; by Nature.com, Global Medical Discovery and National Science Review, 2015, 2:7-8).
    34. Yang L* and Chen LL*. 2014. Microexons go big. Cell, 159(7):1488-1489.
    35. Zhang Y#, Zhang XO#, Chen T, Xiang JF, Yin QF, Xing YH, Zhu S, Yang L* and Chen LL*. 2013. Circular intronic long noncoding RNAs. Mol Cell, 51(6):792-806. (Preview by Bolisetty MT and Graveley BR. Mol Cell, 2013, 51:705-706; Research highlights by Nature, 2013, 501:464, Nature China, 2013, doi:10.1038; the mostdownloaded article of the month in Mol Cell).
    36. Yin QF#, Yang L#, Zhang Y, Xiang JF, Wu YW, Carmichael GG* and Chen LL*. 2012. Long noncoding RNAs with snoRNA ends. Mol Cell, 48(2):219-230. (Cover article; Preview by McCann KL and Baserga SJ. Mol Cell, 2012, 48:155-7; Research highlights by Nat Rev Mol Cell Biol, 2012, 13:686; Five recommendations in F1000 Prime; News coverage in Foundation for Prader-Willi Research; Best of Mol Cell 2012).
    37. Yang L, Duff MO, Graveley BR, Carmichael GG and Chen LL*. 2011. Genomewide characterization of non-polyadenylated RNAs. Genome Biol, 12(2):R16. (Editor's pick; Highly accessed).
    获奖及荣誉:
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    研究组成员:
  • 合影