首页 全所PI名录
  • 惠静毅
  • 研究员,研究组长,博士生导师
  • E-mail: jyhui@@sibcb.ac.cn
  • 实验室主页: 
    个人简介:
  •   1993年复旦大学微生物系毕业,获学士学位。1999年德国Free University Berlin毕业,获硕士学位。2003年德国Justus-Liebig-University Giessen毕业,获博士学位。2008年回国后,任中科院上海生科院生化与细胞所研究员,研究组长。

    社会任职:
  •  
    研究方向:
  • RNA加工的调控与功能
    研究工作:
  •   RNA结合蛋白是一类能够结合单链或双链RNA的蛋白质。越来越多的实验结果表明RNA结合蛋白参与了RNA代谢过程中几乎各个步骤,在各种生命活动中发挥重要功能。RNA结合蛋白编码基因的突变、扩增、缺失或者RNA结合蛋白的表达与性质变化与许多人类疾病密切相关。我们通过采用多学科手段,探索RNA结合蛋白调控基因表达的分子机制以及在人类疾病中这些机制是如何失调的。我们特别关注RNA结合蛋白调控RNA加工的机制与功能。RNA加工是RNA成熟并发挥功能所必不可少的步骤,也是基因表达过程中的一个重要调控层面,在这一方向上的研究内容主要包括:1. RNA结合蛋白调控mRNA可变剪接的分子机理研究;2. RNA结合蛋白在肿瘤发生发展中的机制与功能研究;3. 神经发育紊乱中RNA与染色质相互作用的调控机制研究等。

    承担科研项目情况:
  •  
    代表论著:
    1. Jiang Y*, Fu X*, Zhang Y*, Wang SF, Zhu H, Wang WK, Zhang L, Wu P, Wong CCL, Li J, Ma J, Guan JS, Huang Y#, Hui J#. Rett syndrome linked to defects in forming the MeCP2/Rbfox/LASR complex in mouse models. (2021) Nat Commun, 12, 5767
    2. Pan X, Zhao J, Zhou Z, Chen J, Yang Z, Wu Y, Bai M, Jiao Y, Yang Y, Hu X, Cheng T, Lu Q, Wang B, Li CL, Lu YJ, Diao L, Zhong YQ, Pan J, Zhu J, Xiao HS, Qiu ZL, Li J, Wang Z, Hui J, Bao L, Zhang X. 5'-UTR SNP of FGF13 causes translational defect and intellectual disability. (2021) Elife, 10: e63021
    3. Park E, Jiang Y, Hao L, Hui J, Xing Y. Genetic variation and microRNA targeting of A-to-I RNA editing fine tune human tissue transcriptomes. (2021) Genome Biol, 22, 77
    4. Wang JZ*, Fu X*, Fang Z*, Liu H, Zong FY, Zhu H, Yu YF, Zhang XY, Wang SF, Huang Y, Hui J#. QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer. (2021) J Mol Cell Biol, 13, 347-360
    5. Wang X, Li ZT, Yan Y, Lin P, Tang W, Hasler D, Meduri R, Li Y, Hua MM, Qi HT, Lin DH, Shi HJ, Hui J, Li J, Li D, Yang JH, Lin J, Meister G, Fischer U, Liu MF. LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice. (2020) Mol Cell, 77, 999-1013
    6. Yang XJ, Zhu H, Mu SR, Wei WJ, Yuan X, Wang M, Liu Y, Hui J#, Huang Y#. Crystal structure of a Y-box binding protein 1 (YB-1)-RNA complex reveals key features and residues interacting with RNA. (2019) J Biol Chem, 294, 10998-11010
    7. Müller-McNicoll M, Rossbach O, Hui J, Medenbach J. Auto-regulatory feedback by RNA-binding proteins. (2019) J Mol Cell Biol, 11, 930-939
    8. Wu DR*, Gu KL*, Yu JC, Fu X, Wang XW, Guo WT, Liao LQ, Zhu H, Zhang XS, Hui J#, Wang Y#. Opposing roles of miR-294 and MBNL1/2 in shaping the gene regulatory network of embryonic stem cells. (2018) EMBO Rep 19, pii: e45657
    9. Wang F, Fu X, Chen P, Wu P, Fan XJ, Li N, Zhu H, Jia TT, Ji H, Wang ZF, Wong CCL, Hu RG#, Hui J#. SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling. (2017) Cell Res, 27, 540-558 (highlighted by Fu XD. Both sides of the same coin: Rac1 splicing regulating by EGF signaling. (2017) Cell Res, 27, 455-456)
    10. Yuan H, Li N, Fu D, Ren J, Hui J, Peng J, Liu Y, Qiu T, Jiang M, Pan Q, Han Y, Wang X, Li Q, Qin J. Histone methyltransferase SETD2 modulates alternative splicing to inhibit intestinal tumorigenesis. (2017) J Clin Invest, 127, 3375-3391
    11. Wang F, Song W, Zhao HM, Ma YN, Li YX, Zhai D, Pi JN, Si YM, Xu JY, Dong L, Su R, Zhang MM, Zhu Y, Ren XX, Miao F, Liu WJ, Li FJ, Zhang JW, He AB, Shan G, Hui J, Wang LF, Yu J. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. (2017) Cell Res, 27, 416-439
    12. Li F, Yi P, Pi J, Li L, Hui J, Wang F, Liang A, Yu J. QKI5-mediated alternative splicing of the histone variant macroH2A1 regulates gastric carcinogenesis. (2016) Oncotarget, 7, 32821-32834
    13. Wu SL, Fu X*, Huang J*, Jia TT, Zong FY, Mu SR, Zhu H, Yan Y, Qiu S, Wu Q, Yan W, Peng Y, Chen J, Hui J#. Genome-wide analysis of YB-1-RNA interactions reveals a novel role of YB-1 in miRNA processing in glioblastoma multiforme. (2015) Nucleic Acids Res, 43, 8516-8528
    14. Guo R, Zheng L, Park JW, Lv R, Chen H, Jiao F, Xu W, Mu S, Wen H, Qiu J, Wang Z, Yang P, Wu F, Hui J, Fu X, Shi X, Shi YG, Xing Y, Lan F, Shi Y. BS69/ZMYND11 reads and connects histone H3.3 lysine 36 trimethylation-decorated chromatin to regulated pre-mRNA processing. (2014) Mol Cell, 56, 298-310
    15. Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J#. The RNA-binding protein QKI suppresses cancer-associated aberrant splicing. (2014) PLOS Genet, 10, e1004289
    16. Jiang Y, Hui J#. Chromatin structure and mRNA alternative splicing. (2014) Chem Life, 4, 449-455 (invited review)
    17. Wei WJ*, Mu SR*, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J#. YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts. (2012) Nucleic Acids Res, 40, 8622-8636
    18. Huang Y, Li W, Yao X, Lin QJ, Yin JW, Liang Y, Heiner M, Tian B, Hui J, Wang G. Mediator complex regulates alternative mRNA processing via the MED23 subunit. (2012) Mol Cell, 45, 459-469
    19. Heiner M, Hui J, Schreiner S, Hung LH, Bindereif A. HnRNP L-mediated regulation of mammalian alternative splicing by interference with splice site recognition. (2010) RNA Biol, 7, 56-64
    20. Kishore S, Khanna A, Zhang Z, Hui J, Balwierz PJ, Stefan M, Beach C, Nicholls RD, Zavolan M, Stamm S. The snoRNA MBII-52 (SNORD 115) is processed into smaller RNAs and regulates alternative splicing. (2010) Hum Mol Genet, 19, 1153-1164
    21. Ro?bach O, Hung LH, Schreiner S, Grishina I, Heiner M, Hui J, Bindereif A. Auto- and crossregulation of the hnRNP L proteins by alternative splicing. (2009) Mol Cell Biol, 29, 1442-1451
    22. Hui J#. Regulation of mammalian pre-mRNA splicing. (2009) Sci China C Life Sci, 52, 253-260 (invited review)
    23. Hui J, Kishore S, Khanna A, Stamm S. Analysis of alternative splicing with microarrays. (2009) In Bioinformatics for Systems Biology (Krawetz SA ed.), Humana Press, pp267-279
    24. Hung LH*, Heiner M*, Hui J*, Schreiner S, Benes V, Bindereif A. Diverse roles of hnRNP L in mammalian mRNA processing: a combined microarray and RNAi analysis. (2008) RNA, 14, 284-296
    25. Lorenz M, Hewing B, Hui J, Zepp A, Baumann G, Bindereif A, Stangl V, Stangl K. Alternative splicing in intron 13 of the human eNOS gene: a potential mechanism for regulating eNOS activity. (2007) FASEB J, 21, 1556-1564
    26. Hui J, Bindereif A. Alternative pre-mRNA splicing in the human system: unexpected role of repetitive sequences as regulatory elements. (2005) Biol Chem, 386, 1265-1271 (invited review)
    27. Hui J*, Hung LH*, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. (2005) EMBO J, 24, 1988-1998
    28. Palfi Z, Hui J, Bindereif A. Biotin-based affinity purification of RNA-protein complexes. (2004) In Handbook of RNA Biochemistry, (Westhof E, Bindereif A, Sch?n A, Hartmann RK eds), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, pp676-693
    29. Hui J, Reither G, Bindereif A. Novel function role of CA repeats and hnRNP L in RNA stability. (2003) RNA, 9, 931-936
    30. Hui J, Stangl K, Lane WL, Bindereif A. HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. (2003) Nat Struct Biol, 10, 33-37. (Comment by Bilbao D, Valcárcel J. Getting to the heart of a splicing enhancer. (2003) Nat Struct Biol, 10, 6-7)
    31. Yang J, Hui J, Li G, Wang Y, Yuan H, Li Y. Expression of the recombinant hepatitis B virus surface antigen carrying preS epitopes in Pichia pastoris. (2000) Acta Biochimica et Biophysica Sinica, 32, 39-144
    32. Hui J, Li G, Kong Y, Wang Y. Expression and characterization of chimeric hepatitis B surface antigen particles carrying preS epitopes. (1999) J Biotechnol, 72, 49-59
    33. Hui J, Mancini M, Li G, Wang Y, Tiollais P, Michel ML. (1999) Immunization with a plasmid encoding a modified hepatitis B surface antigen carrying the receptor binding site for hepatocytes. Vaccine, 17, 1711-1718
    34. Hui J, Li G, Kong Y, Wang Y. DNA-mediated immunization against hepatitis B surface antigen carrying preS epitopes. (1999) Chinese Science Bulletin, 44, 620-62
    35. Hui J, Li G, Kong Y, Wang Y. A modified HBsAg containing the preS1 and preS2 epitopes. (1998) Sci China C Life Sci, 41, 56-63
    获奖及荣誉:
  •  
    研究组成员:
  •