刘小龙--中国科学院分子细胞科学卓越创新中心

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刘小龙
研究员，研究组长，博士生导师
E-mail: xiaolongliu@@sibcb.ac.cn
实验室主页:

个人简介：

1994年毕业于南昌大学，获有机化学专业学士学位；1999年毕业于中国科学院上海生物化学研究所，获生物化学与分子生物学专业博士学位；1999年至2004年在美国国立癌症研究所(National Cancer Institute, NIH)接受博士后训练，从事细胞免疫学研究。2004年聘为研究员；2009年入选国家杰出青年科学基金获得者；2014年入选科技部中青年科技创新领军人才。担任国家重点基础研究发展计划(973计划)项目首席(2013年-2017年)，国家自然科学基金创新群体项目负责人(2017年-2022年)。近年来，阐明了免疫细胞的分化成熟及其功能作用机制，创建了基于NK细胞的肿瘤免疫治疗技术，发展了新型RNA递送技术。在Nat Immunol, Cell Biomaterials, JEM, PNAS和EMBO J等学术期刊上发表50余篇研究论文。2008年获得明治乳业生命科学奖；2009年获得第十一届中国青年科技奖；2010年评为中科院优秀研究生指导教师；2011年评为全国优秀博士学位论文指导教师；2012年获得A-IMBN Research Young Investigators Award。担任Cell Res和Cell Mol Immunol 等学术期刊的编委；任中国生物化学与分子生物学会秘书长。

社会任职：

研究方向：

免疫细胞分化与免疫治疗

研究工作：

免疫细胞在骨髓或胸腺中分化成熟，迁移至外周淋巴系统中发挥免疫功能。我们研究组的科研工作以T细胞和NK细胞为主要研究对象，围绕他们的分化成熟与功能调控机制而展开。在T细胞方面，主要研究其定向分化及其在肿瘤免疫调控中的分子细胞作用机制；在NK细胞方面，主要聚焦其功能发挥与失调及功能改造技术，发展基于NK细胞的肿瘤与自免疾病治疗技术；在RNA递送方面，在建立即混即用新型递送载体的基础上，构建高效免疫细胞转染技术以及组织特异性递送方法，发展肿瘤治疗新策略。

承担科研项目情况：

代表论著：

1. Qin Z, Qu Y, Tian W, Tao P, Yang L, Zhao R, Ying Q, Liu H, Lu Y, Jiang Y, Zhuang H, Deng Z, Liu G, Qin W, Liu X*. Tuning the loading position of mRNA onto ionizable neo-liposomes to improve endosomal escape. Cell Biomaterials. 2026(2): 100339
2. Xu Y, Luo H, Wang J, Liu H, Chen L, Ji H, Deng Z*, Liu X*. CD103⁺ T cells eliminate damaged alveolar epithelial type II cells under oxidative stress to prevent lung tumorigenesis. Adv Sci (Weinh). 2025: e2503557
3. Qin Z, Jiang Y, Tao P, Liu H, Zhong H, Ding X, Zhao R, Lu Y, Zhang L, Chen X, Zhuang H, Wu J, Deng Z, Liu X*. A ready-to-use RNA delivery platform modulated by metal ions. Chem Eng J. 2025; 506:e160170
4. Bao W, Xing H, Cao S, Long X, Liu H, Ma J, Guo F, Deng Z*, Liu X*. Neutrophils restrain sepsis associated coagulopathy via extracellular vesicles carrying superoxide dismutase 2 in a murine model of lipopolysaccharide induced sepsis. Nat Commun. 2022; 13(1):4583.
5. Hu C, Chen B, Li Z, Yang T, Xu C, Yang R, Yu P, Zhao J, Liu T, Liu N, Shan B, Zhang Q, Song J, Fei M, Zong L, Zhang J, Wu J, ChenS, Wang Y, Chang B, Hou D, Liu P, Jiang Y, Li X, Chen X, Deng C, Ren Y, Wang R, Jin J, Xue K, Zhang Y, Du M, Shi J, Wu L, Chang C, Shen S, Chen Z, Chen S, Liu X*, Sun X*, Zheng M*, Wang L*. Targeting UHRF1-SAP30-MXD4 axis for leukemia initiating cell eradication in myeloid leukemia. Cell Res. 2022; 32(12):1105-1123.
6. Xu Y, Ma J, Luo H, Shi Y, Liu H, Sun A, Xu C, Ji H, Liu X*. Chromatin assembly factor 1B critically controls the early development but not function acquisition of invariant natural killer T cells in mice. Eur J Immunol. 2021; 51(7):1698-14.
7. Shen H, Gu C, Liang T, Liu H, Guo F, Liu X*. Unveiling the heterogeneity of NKT cells in the liver through single cell RNA sequencing. Sci Rep. 2020; 10(1): 19453.
8. Sun A, Xu K, Liu H, Li H, Shi Y, Zhu X, Liang T, Li X, Cao X, Ji Y, Jiang T, Xu C, Liu X*. The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates. Sci Rep. 2020; 10(1): 4126.
9. Sun X, Cui Y, Feng H, Liu H, Liu X*. TGF-β signaling controls Foxp3 methylation and T reg cell differentiation by modulating Uhrf1 activity. J Exp Med. 2019 Sep 12. pii: jem.20190550. doi: 10.1084/jem.20190550.
10. Xu Y, Sun Y, Shen H, Dai Y, Liu H, Li R, Zhang H, Wu L, Zhu X, Liu X*. Regulation of the terminal maturation of iNKT cells by mediator complex subunit 23. Nat Commun. 2018; 9(1):3875.
11. Chen X, Zhao J, Gu C, Cui Y, Dai Y, Song G, Liu H, Shen H, Liu Y, Wang Y, Xing H, Zhu X, Hao P, Guo F*, Liu X*. Med23 serves as a gatekeeper of the myeloid potential of hematopoietic stem cells. Nat Commun. 2018; 9(1):3746.
12. Zhang R, Ma H, Gao Y, Wu Y, Qiao Y, Geng A, Cai C, Han Y, Zeng YA, Liu X*, Ge G*. Th-POK regulates mammary gland lactation through mTOR-SREBP pathway. PLoS Genet. 2018; 14(2):e1007211.
13. Zhao J, Chen X, Song G, Zhang J, Liu H, Liu X*. Uhrf1 controls the self-renewal versus differentiation of hematopoietic stem cells by epigenetically regulating the cell-division modes. Proc Natl Acad Sci U S A. 2017; 114(2):E142-E151. [Highlighted by Proc Natl Acad Sci U S A. 2017; 114(2):192-94].
14. Chen J, Cai Z, Zhang L, Yin Y, Chen X, Chen C, Zhang Y, Zhai S, Long X, Liu X*, Wang X*. Lis1 Regulates Germinal Center B Cell Antigen Acquisition and Affinity Maturation. J Immunol. 2017;198(11):4304-4311.
15. Cui Y, Chen X, Zhang J, Sun X, Liu H, Bai L, Xu C, Liu X*. Uhrf1 Controls iNKT Cell Survival and Differentiation through the Akt-mTOR Axis. Cell Rep. 2016; 15(2):256-63.
16. Xu K, Liu H, Shi Z, Song G, Zhu X, Jiang Y, Zhou Z, Liu X*. Disruption of the RAG2 zinc finger motif impairs protein stability and causes immunodeficiency. Eur J Immunol. 2016; 46(4):1011-9.
17. Deng Z, Liu H, Rui J, Liu X*. Reconstituted Thymus Organ Culture. Methods Mol Biol. 2016; 1323:151-8.
18. Deng Z, Liu H, Liu X*. RAG1-mediated ubiquitylation of histone H3 is required for chromosomal V(D)J recombination. Cell Res. 2015; 25(2):181-92.
19. Sun Y, Zhu X, Chen X, Liu H, Xu Y, Chu Y, Wang G*, Liu X*. The mediator subunit Med23 contributes to controlling T-cell activation and prevents autoimmunity. Nat Commun. 2014; 5:5225.
20. Zhang Y, Xu K, Deng A, Fu X, Xu A*, Liu X*. An amphioxus RAG1-like DNA fragment encodes a functional central domain of vertebrate core RAG1. Proc Natl Acad Sci U S A. 2014; 111(1):397-402.
21. Chen X, Zhang J, Zhao J, Liu H, Sun X, Zhao M, Liu X*. Lis1 is required for the expansion of hematopoietic stem cells in the fetal liver. Cell Res. 2014; 24:1013-16.
22. Xiao G, Deng A, Liu H, Ge G, Liu X*. Activator protein 1 suppresses antitumor T-cell function via the induction of programmed death 1. Proc Natl Acad Sci U S A. 2012; 109(38):15419-24.
23. Rui J, Liu H, Zhu X, Cui Y, Liu X*. Epigenetic silencing of CD8 genes by ThPOK-mediated deacetylation during CD4 T cell differentiation. J Immunol. 2012; 189(3):1380-90. [Highlighted by J Immunol. 2012; 189(3):1098].
24. Cao Y, Li H, Liu H, Zhang M, Hua Z, Ji H, Liu X*. LKB1 regulates TCR-mediated PLCγ1 activation and thymocyte positive selection. EMBO J. 2011; 30(10):2083-93. [Selected by Faculty of 1000 Biology]
25. Wen X, Liu H, Xiao G, Liu X*. Downregulation of the transcription factor KLF4 is required for the lineage commitment of T cells. Cell Res. 2011; 21(12):1701-10.
26. Zhang M, Zhang J, Rui J, Liu X*. p300-Mediated Acetylation Stabilizes the Th-Inducing POK Factor. J Immunol. 2010; 185(7):3960-9. [Highlighted by J Immunol. 2010; 185(7):3783]
27. Cao Y, Li H, Sun Y, Chen X, Liu H, Gao X and Liu X*. Interferon regulatory factor 4 regulates thymocyte differentiation by repressing Runx3 expression. Eur J Immunol. 2010; 40(11):3198-209.
28. Cao Y, Li H, Liu H, Zheng C, JI H, Liu X*. The serine/threonine kinase LKB1 controls thymocyte survival by regulation of AMPK activation and Bcl-XL expression. Cell Res. 2010; 20:99-108.
29. Zhang Y, Li H, Wang X, Gao X, Liu X*. Regulation of T cell development and activation by creatine kinase B. PLoS One. 2009; 4(4):e5000.
30. Yang Z, Cao Y, Zhu X, Huang Y, Ding Y, Liu X*. Znhit1 causes cell cycle arrest and down-regulates CDK6 expression. Biochem Biophys Res Commun. 2009; 386(1):146-52.
31. Wang X, Zhang Y, Xiao G, Gao X, Liu X*. c-Fos enhances survival of thymocytes during positive selection by upregulating Bcl-2. Cell Res. 2009; 19:340-347.
32. Wang X, Xiao G, Zhang Y, Wen X, Gao X, Okada S, Liu X*. Regulation of Tcrb recombination ordering by c-Fos-dependent RAG deposition. Nat Immunol. 2008; 9(7):794-801. [Research highlighted by Nat Rev Immunol. 2008; 8(7):494]
33. Zhang Y, Yang Z, Cao Y, Zhang S, Li H, Huang Y, Ding YQ, Liu X*. The Hsp40 family chaperone protein DnaJB6 enhances Schlafen1 nuclear localization which is critical for promotion of cell cycle arrest in T cells. Biochem J. 2008; 413(2):239-250.
34. Shi J, Yang Z, Wang M, Cheng G, Li D, Wang Y, Zhou Y, Liu X*, Xu C*. Screening of an antigen target for immunocontraceptives from cross-reactive antigens between human sperm and Ureaplasma urealyticum. Infect Immun. 2007; 75(4):2004-2011.
35. Sun G, Liu X, Mercado P, Jenkinson SR, Kypriotou M, Feigenbaum L, Galéra P, Bosselut R*. The zinc finger protein cKrox directs CD4 lineage differentiation during intrathymic T cell positive selection. Nat Immunol. 2005; 6(4):373-381.
36. Liu X, Taylor BJ, Sun G, Bosselut R*. Analyzing expression of perforin, Runx3, and Thpok genes during positive selection reveals activation of CD8-differentiation programs by MHC II-signaled thymocytes. J Immunol. 2005; 175(7):4465-4474.
37. Liu X, Bosselut R*. Duration of TCR signaling controls CD4-CD8 lineage differentiation in vivo. Nat Immunol. 2004; 5(3):280-288.
38. Liu X, Adams A, Wildt KF, Aronow B, Feigenbaum L, Bosselut R*. Restricting Zap70 expression to CD4+CD8+ thymocytes reveals a T cell receptor-dependent proofreading mechanism controlling the completion of positive selection. J Exp Med. 2003; 197(3):363-373.
39. Liu X, Wu X and Zhou Y*. Identification of key residues responsible for enzymatic and platelet-aggregation-inhibiting activities of acidic phospholipase A2 from Agkistrodon halys Pall. J Nat Toxins. 2001; 10: 43-55.
40. Liu X, Xiong Y, Wu X and Zhou Y*. A comparative study on the structure and function of phospholipase A2s from Agkistrodon acutus. Protein Peptide Lett. 2000; 7: 83-90.
41. Liu X, Pan H., Yang G, Wu X and Zhou Y*. Cloning, Expression and Biochemical Characterization of A Basic-Acidic Hybrid Phospholipase A2-II From Agkistrodon halys Pallas. Biochim Biophys Acta. 1999; 1431: 157-165.

获奖及荣誉：

研究组成员：

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